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To additional explore the function of IDL6 peptides in leaf senescence, dark treatments have been carried out to induce leaf senescence. The specific regulatory mechanisms underlying the interactions between IDL6 and senescence-regulating transcription elements stay to be further studied. These results indicated that transcription elements WRKY53 and CDF4, both have been characterised as optimistic regulators of senescence (Miao et al., 2004; Xu et al., 2020), may operate downstream of IDL6 in regulating leaf senescence. Three days later, the chlorophyll levels of all leaves from the IDL6 OE lines were considerably reduced, whereas comparatively less chlorophyll breakdown occurred within the leaves of idl6 mutant plant In this examine, we discovered that WRKY53 and CDF4 have been down-regulated in the idl6 mutant , whereas they confirmed higher expression in overexpression IDL6 gene line . The 5th, sixth, and seventh leaves were excised from four.5-week-old crops , placed on moistened filter papers and incubated beneath darkness to induce senescence.